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Zinc dry electrodes were fabricated and investigated for wearable electrophysiology recording. Results from electrochemical impedance spectroscopy and electromyography functionality testing show that zinc electrodes are suitable for use in electrophysiology. Two electrode configurations were tested: a standard disc and a custom tripolar concentric ring configuration. However, no functional benefit was observed with the tripolar concentric ring electrodes as compared to the disc electrodes.Zinc, Electrodes, Concentric Ring Electrodes, EMG, Biosensing 

Abstract Human cortical organoids, three-dimensional neuronal cultures, are emerging as powerful tools to study brain development and dysfunction. However, whether organoids can functionally connect to a sensory network in vivo has yet to be demonstrated. Here, we combine transparent microelectrode arrays and two-photon imaging for longitudinal, multimodal monitoring of human cortical organoids transplanted into the retrosplenial cortex of adult mice. Two-photon imaging shows vascularization of the transplanted organoid. Visual stimuli evoke electrophysiological responses in the organoid, matching the responses from the surrounding cortex. Increases in multi-unit activity (MUA) and gamma power and phase locking of stimulus-evoked MUA with slow oscillations indicate functional integration between the organoid and the host brain. Immunostaining confirms the presence of human-mouse synapses. Implantation of transparent microelectrodes with organoids serves as a versatile in vivo platform for comprehensive evaluation of the development, maturation, and functional integration of human neuronal networks within the mouse brain.